Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
Highlights
Performs under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
Note
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
CategoryName
Concentration
40 U/µL
Definition of Activity Unit
One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
Hazardous
No
Hazardous:
No
Inactivation
Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
Inhibition
Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight
49.6 kDa monomer
Quality Control
The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
Functionally tested in RNA and cDNA synthesis.
Shelf Life:
Shipping Condition:
Shipping Information
Source
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
SpecificationName
SpecificationValue
Storage Buffer
The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition
-20 C
Storage Condition:
fermentasRNA酶抑制劑RiboLock RNase Inhibitor *
*明細：
DreamTaq Green PCR Master Mix (2×)
K1081
200rxns
500
300
K1082
1000rxns
2,000
1200
DreamTaq PCR Master Mix (2×)
K1071
200rxns
500
300
K1072
1000rxns
2,000
1200
PCR master Mix (2×)
K0171
200rxns
450
276
K0172
1000rxns
1,800
1080
Long PCR Enzyme Mix
K0181
100U
250
150
K0182
500U
1,000
600
DreamTaq DNA Polymerase
EP0701
200U
150
90
EP0702
500U
200
120
EP0703
5x500U
900
540
EP0704
20x500U
3,200
1920
DreamTaq Green DNA Polymerase
EP0711
200U
150
90
EP0712
500U
200
120
EP0713
5x500U
1,080
648
EP0714
20x500U
3,200
1920
TurboFect in vitro Transfection Reagent
R0531
1ml
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1300
TurboFect siRNA Transfection Reagent
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TurboFect Protein Transfection Reagent
R1411
125ul
2,140
1391
TurboFect in vivo Transfection Reagent
R0541
120ul
3,000
1950
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