国产区一二-久久久看片-日韩久久中文字幕-久久精品伊人-av免费看网站-一本大道久久香蕉成人网-欧美精品亚洲日韩aⅴ-五月激情av-成人久久久精品国产乱码一区二区-久久综合精品视频-欧美日韩www-超碰2022-色欲来吧来吧天天综合网-久久久日韩精品一区二区三区-在线观看www.-成人亚洲a片v一区二区三区动漫-亚洲女同性同志熟女-中日韩va无码中文字幕-国产精品一区二区熟女不卡-夜色88v精品国产亚洲

sigmaDL-二硫蘇糖醇DL-Dithiothreitol

CAS number: 3483-12-3
Product Description:
Appearance: White Powder
Molecular formula: C4H10O2S2
Melting Point: 42-43°C
Molecular weight: 154.1 g/mol
Solubility: 0.1 M in H2O, 20°C, complete, colorless
pH: 4.0-6.5 （0.1 M in H2O, 25°C）
This product is designated as BioChemika Ultra for Molecular Biology grade and is suitable for applications in the molecular biology, specially if high purity is required. It has been analyzed for low contaminating impurity and the absence of nucleases.
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 （1949）
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB （Fluka 43760）.2
Applications:
It is used in buffers for isolation of RNA5 or buffers used for RT-PCR starting with mRNA6,7.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether.
Solutions oxidize relatively slowly in air.3 It is recommended to make solutions fresh daily. Recorded half-life （hours） of DTT solutions at various pH and temperatures are as follows:4
Conditions （all in 0.1 M KPO4 buffer）
Half life （Hours）
pH 6.5 at 20°C
40
pH 7.5 at 20°C
10
pH 8.5 at 20°C
1.4
pH 8.5 at 0°C
11
pH 8.5 at 40°C
0.2
pH 8.5 at 20°C +0.1 mM Cu2+
0.6
pH 8.5 at 20°C +0.1 mM EDTA
4
Storage/Stability:
Storage Temperature: 4°C
When stored as indicated, this product is stable for years.

上海力敏實業有限公司代理sigma產品，咨詢sigmaDL-二硫蘇糖醇DL-Dithiothreitol 詳細產品信息